The elementary bodies of the Chlamydia trachomatis stains green inside the red host cell, and the presence of more elementary bodies in a field of view compared to the positive control is considered a positive result. 4. How would the results be affected if a negative control gave a positive result? If a negative control gave a positive result, the positive results would be underestimated. A C T I V I T Y 2 Comparing Samples with Ouchterlony Double Diffusion 1. Describe how you were able to determine what antigen is in the unknown well. The results between well 2 & 5 were the same results as.
We know that human serum albumin was placed in 4, so it should be the same in 5 to achieve the same result. 2. Why does the precipitin line form? The precipitin line occurs when the antigen and antibody are in optimal proportions and cross-linking occurs forming an insoluble precipitate. 3. Did you think human serum albumin and bovine serum albumin would have epitopes in common? How well did the results compare with your prediction? I think they have epitopes in common for the results showed they have partial identity. Same as my prediction.